Isoelectric Focusing

Now equipped with rehydration and focusing trays, the redesigned AC-IEF has been optimized to perform first-dimension isoelectric focusing (IEF) with IPG (immobilized pH gradient) strips quickly, easily and reproducibly.

An ideal entry-level system for both inexperienced and occasional IEF users, the AC-IEF is also versatile enough to meet the needs of laboratories with increased throughput requirements.

                               Features include:

• A high-capacity focusing tray that accommodates up to twelve IPG strips

• Adjustable ‘pick-and-place’ electrodes clip conveniently anywhere within the focusingtray to resolve IPG strips 7-24cm in length ;colour-coded to prevent polarity reversal

• A cooling plate, manufactured from a special grade ceramic in a large 26x26cmsurface area, facilitates effective heat dissipation and homogenous thermal control, particularly during high voltage IEF techniques

• An optional, but recommended ACCULAB Scientific recirculating chiller connects quickly and easily to the cooling

plate via snap-lock connectors to maintainoptimal operating temperatures for IPG strips (20°C) and precast gels(4°C)

• Rehydration tray allows convenient transfer of IPG strips to the focusing tray without time-consuming removal of residual rehydration buffer; also enables focusing tray to remain permanently in use for IEF tomaximise throughput, and provides useful storage at -20°C for focused strips before second-dimension runs

• Electrode frame clips directly on to the cooling plate and includes adjustable electrodes to run horizontal precast IE Fand PAGE gels Power Supply Option– Consort EV232

• 3000V, 150mA, 150W power supply -enables desired Volt-hours for focusing tobe attained faster at maximum voltage

• Nine different program settings with nine different parameters and voltage ramping make the EV232 ideal for multi-step IEFtechniques

• Data-logging capacity for up to 3600 outputvalues, while run data may be collected viaRS232 connection and free downloadabledata acquisition software

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